Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC660.05
Gene ID 03/E12
Gene synonyms/obsolete
Gene product glycine rich protein; WW domain; paralog?
Entry clone Cloned
ORF length (unspliced) 432
ORF length (spliced)
Entry clone length 432
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments ApaI should be used instead of NotI for integration using pDUAL.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC660.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTGTTTATCAAACTTA
Rev primer name SPBC660.05.Rv
Rev primer SEQ AGAAAGCTGGGTATCTGCGGCCACGTCCACCA
Amino acid length 143
Molecular weight 15.1
Isoelectric point (calc.) 10.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation Confocal

Image information
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