Gene name |
SPCC1322.15 |
Gene ID |
01/H10 |
Gene synonyms/obsolete |
rpl3402; rpl34-2 |
Gene product |
60S ribosomal protein
L34 |
Entry clone |
Cloned# |
ORF length (unspliced) |
336 |
ORF length (spliced) |
|
Entry clone length |
336 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC1322.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTCAAAGAGTTACTTA |
Rev primer name |
SPCC1322.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTTTGAGACATCTGCTTC |
Amino acid length |
111 |
Molecular weight |
12.8 |
Isoelectric point (calc.) |
11.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol;
nucleolus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to: nucleolus;
nucleus>cytosol; cytosol=nucleus |
Microscope used for
observation |
DeltaVision |