Gene name |
SPAC27D7.05c |
Gene ID |
01/G03 |
Gene synonyms/obsolete |
apc14 |
Gene product |
anaphase-promoting
complex (APC); involved in spore wall assembly; Mei4p
dependent expression; no apparent orthologs |
Entry clone |
Cloned |
ORF length (unspliced) |
324 |
ORF length (spliced) |
|
Entry clone length |
324 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
15T:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC27D7.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATCCGTTTGCTGGTAC |
Rev primer name |
SPAC27D7.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACCATTCAGCCAACTGGTCA |
Amino acid length |
107 |
Molecular weight |
12.1 |
Isoelectric point (calc.) |
9.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
a few moving dots;
aggregates by over expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |