Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC7D4.07c
Gene ID 01/F01
Gene synonyms/obsolete trx1; trx; (trx2)
Gene product thioredoxin I; similar to Sp trx1; involved in stress response
Entry clone Cloned
ORF length (unspliced) 312
ORF length (spliced)
Entry clone length 312
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC7D4.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTGAAACAAGTCTCCGA
Rev primer name SPAC7D4.07.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGGTTCGCCTTAATAGAA
Amino acid length 103
Molecular weight 11.2
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 2 images) See all images

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