| Gene name |
SPAC27E2.11c |
| Gene ID |
01/B09 |
| Gene synonyms/obsolete |
|
| Gene product |
Sp specific families;
hypothetical protein; possibly Sp specific; GPI anchored
protein (pers. comm. Birgit Eisenhaber); glycoprotein; no
apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
246 |
| ORF length (spliced) |
|
| Entry clone length |
246 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
21C:T / 78T:C /
233T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC27E2.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTACTCTCGTGTTCTTTC |
| Rev primer name |
SPAC27E2.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGAGAGACATGACAGAGCAA |
| Amino acid length |
81 |
| Molecular weight |
7.9 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
2 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |