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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11D3.01c
Gene ID 01/B06
Gene synonyms/obsolete
Gene product fungal specific; similar to Nc DUF338; possibly involved in sporulation, cell wall synthesis; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 240
ORF length (spliced)
Entry clone length 240
No. of intron 0
Sequence status Finished
Sequence results 87A:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC11D3.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTAATCCTGGAAACGT
Rev primer name SPAC11D3.01.Rv
Rev primer SEQ AGAAAGCTGGGTACTCGAGGTTTTCTAACTCC
Amino acid length 79
Molecular weight 8.7
Isoelectric point (calc.) 5.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 1 images) See all images

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