Novel gene assay methodology using affinity bioprobes


A novel concept for separating multiple targets, a probe-regulated simultaneous separation (PRESS), was successfully developed using capillary electrophoresis. PRESS methodology is based on the fact that target genes were hybridized with corresponding probes, and resulting complexes were detected at different migration times. We found that the electrophoretic mobility of DNA-grafted conjugate-probes were different, depending on their molar fraction of DNA. We successfully separated two target DNAs having the same chain length using two DNA-grafted conjugate-probes.

Novel gene assay methodology using affinity bioprobes

Novel gene assay methodology using affinity bioprobes

 

Reference

(1)

Murakami Y. and Maeda M.: "Novel Gene Assay by Probe-Regulated Simultaneous Separation Using Capillary Electrophoresis (CE-PRESS)" J. Biosci. Bioeng. (2003) 96, 95-97 (2003).


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