LOCUS 8558 bp ds-DNA circular 19-APR-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 242..616 /ApEinfo_label=lys1 5'-fragment /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=#400080 CDS 6742..6759 /ApEinfo_label=N-terminal hexahistidine tag /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 promoter 5545..6713 /ApEinfo_label=nmt1 promoter /ApEinfo_fwdcolor=#ff6fcf /ApEinfo_revcolor=#ff6fcf misc_feature complement(8513..8519) /ApEinfo_label=Gateway Recomb2 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 CDS 619..956 /ApEinfo_label=lys1 3'-fragment /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=#400080 CDS 6763..6786 /ApEinfo_label=FLAG epitope(1) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 misc_feature complement(8506..8526) /ApEinfo_label=AttB2 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd misc_feature 6831..6837 /ApEinfo_label=Gateway Recomb1 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 CDS 1342..2151 /ApEinfo_label=kanMX6 /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=#400080 CDS 6793..6816 /ApEinfo_label=FLAG epitope(N2) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 misc_feature 6824..6844 /ApEinfo_label=AttB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd CDS 3674..4333 /ApEinfo_label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow CDS 7056..7715 /ApEinfo_label=CmR /ApEinfo_fwdcolor=#800040 /ApEinfo_revcolor=#800040 CDS 8150..8359 /ApEinfo_label=ccdB ORF /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow terminator 1..237 /ApEinfo_label=ADH1 terminator /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 rep_origin 2634..3089 /ApEinfo_label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 4431..5113 /ApEinfo_label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 ORIGIN 1 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 61 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 121 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG 181 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 241 AGTACTTTGT AGTACCTGCG CGGTCTCAAG ATCAAACATT TTTGGAATCG CAAAAAGATG 301 TCATTCCAGC CGGACGTGGC ATGAAGAATG TCCAACTTTT GGTGATCAAT CGTTTTGATA 361 CTAACAAGAT ATGCGGTATC GGAGAAGTTG GTGAAATATA TCTTCGTGCT GGTGGATTGG 421 CTGAGGGCTA CCTTGGAAAT GATGAATTGA CTAGCAAAAA ATTCTTAAAG AGTTGGTTTG 481 CTGATCCCTC CAAGTTTGTA GACCGAACTC CAGAAAATGC GCCCTGGAAA CCTTATTGGT 541 TCGGAATACG CGATCGTATG TATCGTTCTG GTGACTTGGG CCGCTATCTC CCAACTGGTA 601 ATGTAGAATG TAGCGGCCGC CGACGATCAA ATTAAAATTC GTGGTTTCCG AATTGAGTTA 661 GGTGAAATTA ATACCCATCT TTCTCGTCAT CCTAATGTCC GTGAAAATAT TACCCTTGTT 721 CGACGTGATA AGGATGAAGA ACCCACGCTA GTTGCATACA TTGTACCTCA AGGTTTAAAT 781 AAAGATGACT TTGATAGCGC TACTGAGTCG GAAGATATTG TCGTCAATGG TTTGAAGAAG 841 TACAGGAAGC TTATACATGA TATACGGGAA TATCTTAAGA CTAAACTTCC TAGTTATGCT 901 ATTCCTTCTG TAATTGTTCC CCTTCATAAA ATGCCTCTCA ATCCTAATGG AAAGATCTGT 961 TTAGCTTGCC TCGTCCCCGC CGGGTCACCC GGCCAGCGAC ATGGAGGCCC AGAATACCCT 1021 CCTTGACAGT CTTGACGTGC GCAGCTCAGG GGCATGATGT GACTGTCGCC CGTACATTTA 1081 GCCCATACAT CCCCATGTAT AATCATTTGC ATCCATACAT TTTGATGGCC GCACGGCGCG 1141 AAGCAAAAAT TACGGCTCCT CGCTGCAGAC CTGCGAGCAG GGAAACGCTC CCCTCACAGA 1201 CGCGTTGAAT TGTCCCCACG CCGCGCCCCT GTAGAGAAAT ATAAAAGGTT AGGATTTGCC 1261 ACTGAGGTTC TTCTTTCATA TACTTCCTTT TAAAATCTTG CTAGGATACA GTTCTCACAT 1321 CACATCCGAA CATAAACAAC CATGGGTAAG GAAAAGACTC ACGTTTCGAG GCCGCGATTA 1381 AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA 1441 TCAGGTGCGA CAATCTATCG ATTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA 1501 CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG 1561 ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG 1621 TTACTCACCA CTGCGATCCC CGGCAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT 1681 TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT 1741 GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA 1801 ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT 1861 GAACAAGTCT GGAAAGAAAT GCATAAGCTT TTGCCATTCT CACCGGATTC AGTCGTCACT 1921 CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT 1981 GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC 2041 CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT 2101 CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGTACTG 2161 ACAATAAAAA GATTCTTGTT TTCAAGAACT TGTCATTTGT ATAGTTTTTT TATATTGTAG 2221 TTGTTCTATT TTAATCAAAT GTTAGCGTGA TTTATATTTT TTTTCGCCTC GACATCATCT 2281 GCCCAGATGC GAAGTTAAGT GCGCAGAAAG TAATATCATG CGTCAATCGT ATGTGAATGC 2341 TGGTCGCTAT ACTGCTGTCG ATTCGATACT AACGCCGCCA TCCAGTTTAA ACGAGCTCGA 2401 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 2461 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 2521 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC 2581 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC 2641 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT 2701 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC 2761 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT 2821 ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC 2881 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT 2941 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT 3001 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 3061 TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA 3121 TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC 3181 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG 3241 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT 3301 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 3361 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 3421 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 3481 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 3541 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 3601 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 3661 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 3721 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 3781 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 3841 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 3901 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 3961 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 4021 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 4081 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 4141 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 4201 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 4261 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 4321 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 4381 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 4441 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 4501 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 4561 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 4621 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 4681 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 4741 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 4801 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 4861 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 4921 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4981 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 5041 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 5101 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 5161 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 5221 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 5281 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 5341 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 5401 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 5461 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG 5521 CCTGCAGGTC GATCGACTCT AGAGGATCAG AAAATTATCG CCATAAAAGA CAGAATAAGT 5581 CATCAGCGGT TGTTTCATTT CCTATATTTT TTTTTTATTT TTTTATTTTT TAATAAGGGA 5641 AAATTTAACG TCTAAGGATA CAGAAGATTG TTAGCACATT AAAGTAATAA AGGCTTAAGT 5701 AGTAAGTGCC TTAGCATGTT ATTGTATTTC AAAGGACATA ATCTAAAATA ATAACAATAT 5761 CATTTCTCAC AAGTTATTCA ATTTTCTTTT TTTTTTCTAA TAATATCAAG AATGTATTAT 5821 TTGTTTGACA TAAGTCAACT AATTTATTTA ATATGCTGGA TTAATCTTGC AGACATGTAA 5881 ATTAACAAGT TTTAGTCAAA TAACGTTGAA GTTTCAATGA ACTCAAATAA TTTCTCTTTT 5941 TTTTTATATA ACTCTAATCT GATTTATATT TTCCGCAGGG ATCAACTGAA GTTATGACAT 6001 TTGGATTGGA TCACTTATAA CCTTGGTCGC CAAATAATAC AAAAATCAGC GTTATAAAAC 6061 AAAGAAGGTT TTTGTTAAGA AATTAATCCT CTTTCTTGAT AAGAAAGTTG AACCGAAATT 6121 GCAGATACTG ATATATGAAA ATAATACCCA CAATTTTGGG AATAGCGCAA GCCTCAATTT 6181 AAACAATAGG TGAGGACACA TGATAATGAC CTCAATGATT GTTAGAAGAA AAGAGCCTCA 6241 TTACAAAATC GAAAAATGAA TGGTTGGGTA CAAGTTTCCA AAACATGGTA AAGTGGACTT 6301 TGCGTATGAG ACGTAAATAG AAAAAAACAC TTGTTATATG TTTTCTAGAA TTATTGTTGT 6361 CTCTTTATGG TTGGATGATG CAAAATAGTA ATTTCGGTTA GTTGCTGTAA AACACCACGA 6421 GACAAATAGA TATGGATATT TATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG 6481 TTCAGTCACC CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT 6541 CCGATTGTCA TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG 6601 AAACATTGAA GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA 6661 ATTAATTATT TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG 6721 TCGACAGGCC TGGATCCTAT GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC 6781 GATAAACCCA TGGACTACAA GGACGACGAT GACAAAGATA TCACAAGTTT GTACAAAAAA 6841 GCTGAACGAG AAACGTAAAA TGATATAAAT ATCAATATAT TAAATTAGAT TTTGCATAAA 6901 AAACAGACTA CATAATACTG TAAAACACAA CATATCCAGT CACTATGGCG GCCGCATTAG 6961 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATAA TGTGTGGATT TTGAGTTAGG 7021 ATCCGGCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA 7081 CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG 7141 CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA 7201 AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG 7261 CTCATCCGGA ATTCCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC 7321 ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT 7381 ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG 7441 AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC 7501 CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC 7561 CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA 7621 TTCAGGTTCA TCATGCCGTC TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC 7681 AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAAACGCG TGGATCCGGC TTACTAAAAG 7741 CCAGATAACA GTATGCGTAT TTGCGCGCTG ATTTTTGCGG TATAAGAATA TATACTGATA 7801 TGTATACCCG AAGTATGTCA AAAAGAGGTG TGCTATGAAG CAGCGTATTA CAGTGACAGT 7861 TGACAGCGAC AGCTATCAGT TGCTCAAGGC ATATATGATG TCAATATCTC CGGTCTGGTA 7921 AGCACAACCA TGCAGAATGA AGCCCGTCGT CTGCGTGCCG AACGCTGGAA AGCGGAAAAT 7981 CAGGAAGGGA TGGCTGAGGT CGCCCGGTTT ATTGAAATGA ACGGCTCTTT TGCTGACGAG 8041 AACAGGGACT GGTGAAATGC AGTTTAAGGT TTACACCTAT AAAAGAGAGA GCCGTTATCG 8101 TCTGTTTGTG GATGTACAGA GTGATATTAT TGACACGCCC GGGCGACGGA TGGTGATCCC 8161 CCTGGCCAGT GCACGTCTGC TGTCAGATAA AGTCTCCCGT GAACTTTACC CGGTGGTGCA 8221 TATCGGGGAT GAAAGCTGGC GCATGATGAC CACCGATATG GCCAGTGTGC CGGTCTCCGT 8281 TATCGGGGAA GAAGTGGCTG ATCTCAGCCA CCGCGAAAAT GACATCAAAA ACGCCATTAA 8341 CCTGATGTTC TGGGGAATAT AAATGTCAGG CTCCCTTATA CACAGCCAGT CTGCAGGTCG 8401 ACCATAGTGA CTGGATATGT TGTGTTTTAC AGTATTATGT AGTCTGTTTT TTATGCAAAA 8461 TCTAATTTAA TATATTGATA TTTATATCAT TTTACGTTTC TCGTTCAGCT TTCTTGTACA 8521 AAGTGGTGAT ATCATGAGAT CTCGACTCTA GAGGATCC //