LOCUS 8647 bp ds-DNA circular 14-JUL-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 242..488 /ApEinfo_label=his3 5'-fragment /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff CDS 493..808 /ApEinfo_label=his3 3'-fragment /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff CDS 1196..2005 /ApEinfo_label=kanMX6 /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=#400080 CDS 6380..7039 /ApEinfo_label=CmR /ApEinfo_fwdcolor=#800040 /ApEinfo_revcolor=#800040 CDS 7859..8575 /ApEinfo_label=CFP /ApEinfo_fwdcolor=#66ccff /ApEinfo_revcolor=#66ccff CDS 8582..8605 /ApEinfo_label=FLAG epitope(C2) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 CDS 8606..8623 /ApEinfo_label=C-terminal hexahistidine tag /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 terminator 1..237 /ApEinfo_label=ADH1 terminator /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 promoter 5376..6114 /ApEinfo_label=tub1 promoter /ApEinfo_fwdcolor=#ff6fcf /ApEinfo_revcolor=#ff6fcf misc_feature 2247..5375 /ApEinfo_label=pUC119 /ApEinfo_fwdcolor=#b3b3b3 /ApEinfo_revcolor=#b3b3b3 misc_feature complement(7830..7850) /ApEinfo_label=AttB2 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd misc_feature 6148..6168 /ApEinfo_label=AttB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd CDS 7474..7683 /ApEinfo_label=ccdB ORF /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow misc_feature complement(7837..7843) /ApEinfo_label=Gateway Recomb2 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 misc_feature 6155..6161 /ApEinfo_label=Gateway Recomb1 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 ORIGIN 1 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 61 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 121 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG 181 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 241 AGTGTTGGCA GCGATGAAAT TATTGACTCC TTAATTCGCA TTTCTTGTAT TCCTGGAAAA 301 GATAAGATTT TGATGTGTCC ACCTTCGTAC GGAATGTATA CGGTCTCTGC AAAAATCAAC 361 GATGTTGAGG TTGTTAAGGT TCTCCTTGAA CCAGATTTCA ATTTGAACGT CGATGCTATT 421 TGCGAAACAC TTTCTAAAGA TAGTGCCATC AAAGTCTTTT TTGCTTGTTC CCCTGGTAAT 481 CCAACAGCGG CCGCTCTTAA ACTCGAGGAT ATTAAGAAGA TTTTGGAGCA CCCTACATGG 541 AATGGAATCG TTGTCGTCGA TGAGGCCTAT ATTGATTTCT CTGCTCCCGA TATGTCTGCC 601 TTAACTCTTG TCAATGAATA TCCAAACCTT GCTGTTTGCC AAACCCTTTC GAAATCTTTC 661 GGTCTAGCAG GAATTCGGTA AGTTAATCTA AGCTTTTAAG CATTAATATT ATTTCTTTAC 721 TAACTATGAT GTTTAGTATC GGATTTTGTT TGACAAGTAA ACCTATTGCT ACCATTATGA 781 ACTCATTAAA GGCTCCTTAT AACATTAGAT CTGTTTAGCT TGCCTCGTCC CCGCCGGGTC 841 ACCCGGCCAG CGACATGGAG GCCCAGAATA CCCTCCTTGA CAGTCTTGAC GTGCGCAGCT 901 CAGGGGCATG ATGTGACTGT CGCCCGTACA TTTAGCCCAT ACATCCCCAT GTATAATCAT 961 TTGCATCCAT ACATTTTGAT GGCCGCACGG CGCGAAGCAA AAATTACGGC TCCTCGCTGC 1021 AGACCTGCGA GCAGGGAAAC GCTCCCCTCA CAGACGCGTT GAATTGTCCC CACGCCGCGC 1081 CCCTGTAGAG AAATATAAAA GGTTAGGATT TGCCACTGAG GTTCTTCTTT CATATACTTC 1141 CTTTTAAAAT CTTGCTAGGA TACAGTTCTC ACATCACATC CGAACATAAA CAACCATGGG 1201 TAAGGAAAAG ACTCACGTTT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA 1261 TGGGTATAAA TGGGCTCGCG ATAATGTCGG GCAATCAGGT GCGACAATCT ATCGATTGTA 1321 TGGGAAGCCC GATGCGCCAG AGTTGTTTCT GAAACATGGC AAAGGTAGCG TTGCCAATGA 1381 TGTTACAGAT GAGATGGTCA GACTAAACTG GCTGACGGAA TTTATGCCTC TTCCGACCAT 1441 CAAGCATTTT ATCCGTACTC CTGATGATGC ATGGTTACTC ACCACTGCGA TCCCCGGCAA 1501 AACAGCATTC CAGGTATTAG AAGAATATCC TGATTCAGGT GAAAATATTG TTGATGCGCT 1561 GGCAGTGTTC CTGCGCCGGT TGCATTCGAT TCCTGTTTGT AATTGTCCTT TTAACAGCGA 1621 TCGCGTATTT CGTCTCGCTC AGGCGCAATC ACGAATGAAT AACGGTTTGG TTGATGCGAG 1681 TGATTTTGAT GACGAGCGTA ATGGCTGGCC TGTTGAACAA GTCTGGAAAG AAATGCATAA 1741 GCTTTTGCCA TTCTCACCGG ATTCAGTCGT CACTCATGGT GATTTCTCAC TTGATAACCT 1801 TATTTTTGAC GAGGGGAAAT TAATAGGTTG TATTGATGTT GGACGAGTCG GAATCGCAGA 1861 CCGATACCAG GATCTTGCCA TCCTATGGAA CTGCCTCGGT GAGTTTTCTC CTTCATTACA 1921 GAAACGGCTT TTTCAAAAAT ATGGTATTGA TAATCCTGAT ATGAATAAAT TGCAGTTTCA 1981 TTTGATGCTC GATGAGTTTT TCTAATCAGT ACTGACAATA AAAAGATTCT TGTTTTCAAG 2041 AACTTGTCAT TTGTATAGTT TTTTTATATT GTAGTTGTTC TATTTTAATC AAATGTTAGC 2101 GTGATTTATA TTTTTTTTCG CCTCGACATC ATCTGCCCAG ATGCGAAGTT AAGTGCGCAG 2161 AAAGTAATAT CATGCGTCAA TCGTATGTGA ATGCTGGTCG CTATACTGCT GTCGATTCGA 2221 TACTAACGCC GCCATCCAGT TTAAACGAGC TCGAATTCAC TGGCCGTCGT TTTACAACGT 2281 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC 2341 GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC 2401 CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA 2461 CACCGCATAC GTCAAAGCAA CCATAGTACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG 2521 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT 2581 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC 2641 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG 2701 ATTTGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA 2761 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC 2821 CTATCTCGGG CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA 2881 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGTTTACAA 2941 TTTTATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC 3001 ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA 3061 GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA 3121 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA 3181 TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT 3241 GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 3301 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA 3361 TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG 3421 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA 3481 GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA 3541 AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 3601 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC 3661 TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA 3721 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC 3781 ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA 3841 TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 3901 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG 3961 CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG 4021 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG 4081 GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC 4141 GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC 4201 AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT 4261 AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 4321 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC 4381 GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG 4441 ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA 4501 ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC 4561 CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 4621 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA 4681 CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC 4741 TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC 4801 CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT 4861 GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 4921 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC 4981 TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG 5041 ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC 5101 GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG 5161 CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA 5221 GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT 5281 TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA 5341 ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCATCCT CATTCTTTGG AGCCCTTTCA 5401 CTTGTTTTCA ACTTATACAC TGTACTCATT TTTTTTAAAT CGTTTTATAA AAGCCTAATA 5461 ACAGTGTTCC AAATAAATCA AAACTTTTTC CGCGTAACTC TAGTATAAGC CGGAACAAGG 5521 CTAAGAAACA AATAAATGCA ACGTTTTTAT ATTTCGATCT ATCAAATCCT CAATTACGGA 5581 AGGGCAATTA GTAGCGACAC AAGAGATATA CGCTGCTGAC AAGTTTTTCG TCGTAATAAA 5641 AGAGCTTTTG AAGAGAAAAA AATTACGGCG GAGCGTTGCA ATTCGTTATA TATAATGTGC 5701 TATGCATAAA CTCTACTTTT GCTAGGAATT CTCCTTGCCC TTATGTAATA TTTTATATAC 5761 TTTTAAAAAG ATTTACATGA GGATATACCT GTTCTATAAA GGAGCACATG TTGAGTTTTA 5821 ATGGAAATTT ACCAATCCAA TCTTTCACTT ACAAATTGAG AAAATAAAAG GTGAAATTGA 5881 TGAAGATCCT AATCTTTTTA TGATTAGAAA GCTTAAAACA CCTGACTGAG TAAGCTTCTT 5941 TTTGCAAGCA ATGCATTATT TTAATGTCTA TTTACATGTT TAGCATACTT CCTTCCGTAT 6001 CATCAATAAA TATCGTTAGA CTTCTTTATT AAGGAATTGT TTTGATTAAG TGTACACCGC 6061 ACTTCACTAC AAACCAAACA CCAAAAACCT CGTAGAAACC CTGGAAAGAC GTTCGCTAGC 6121 GTCGACAGGC CTGGATCCGG ATATCAACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT 6181 AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT 6241 ACTGTAAAAC ACAACATATC CAGTCACTAT GGCGGCCGCA TTAGGCACCC CAGGCTTTAC 6301 ACTTTATGCT TCCGGCTCGT ATAATGTGTG GATTTTGAGT TAGGATCCGG CGAGATTTTC 6361 AGGAGCTAAG GAAGCTAAAA TGGAGAAAAA AATCACTGGA TATACCACCG TTGATATATC 6421 CCAATGGCAT CGTAAAGAAC ATTTTGAGGC ATTTCAGTCA GTTGCTCAAT GTACCTATAA 6481 CCAGACCGTT CAGCTGGATA TTACGGCCTT TTTAAAGACC GTAAAGAAAA ATAAGCACAA 6541 GTTTTATCCG GCCTTTATTC ACATTCTTGC CCGCCTGATG AATGCTCATC CGGAATTCCG 6601 TATGGCAATG AAAGACGGTG AGCTGGTGAT ATGGGATAGT GTTCACCCTT GTTACACCGT 6661 TTTCCATGAG CAAACTGAAA CGTTTTCATC GCTCTGGAGT GAATACCACG ACGATTTCCG 6721 GCAGTTTCTA CACATATATT CGCAAGATGT GGCGTGTTAC GGTGAAAACC TGGCCTATTT 6781 CCCTAAAGGG TTTATTGAGA ATATGTTTTT CGTCTCAGCC AATCCCTGGG TGAGTTTCAC 6841 CAGTTTTGAT TTAAACGTGG CCAATATGGA CAACTTCTTC GCCCCCGTTT TCACCATGGG 6901 CAAATATTAT ACGCAAGGCG ACAAGGTGCT GATGCCGCTG GCGATTCAGG TTCATCATGC 6961 CGTCTGTGAT GGCTTCCATG TCGGCAGAAT GCTTAATGAA TTACAACAGT ACTGCGATGA 7021 GTGGCAGGGC GGGGCGTAAA GATCTGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC 7081 GTATTTGCGC GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT 7141 GTCAAAAAGA GGTGTGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT 7201 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA 7261 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG 7321 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GACTGGTGAA 7381 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA 7441 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT 7501 CTGCTGTCAG ATAAAGTCTC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC 7561 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG 7621 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA 7681 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT 7741 ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT 7801 GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TTGATATCAT 7861 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG 7921 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG 7981 CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT 8041 CGTGACCACC CTGACCTGGG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA 8101 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT 8161 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT 8221 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA 8281 GCTGGAGTAC AACTACATCA GCCACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG 8341 CATCAAGGCC AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA 8401 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA 8461 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT 8521 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGCCCAT 8581 GGACTACAAG GACGACGATG ATAAACATCA CCACCATCAC CACTGAGATC TCGACTCTAG 8641 AGGATCC //