LOCUS 8758 bp ds-DNA circular 19-APR-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 241..552 /ApEinfo_label=arg1 5'-fragment /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff CDS 8663..8686 /ApEinfo_label=FLAG epitope(1) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 misc_feature complement(8631..8651) /ApEinfo_label=AttB2 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd CDS 557..894 /ApEinfo_label=arg1 3'-fragment /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff CDS 7181..7840 /ApEinfo_label=CmR /ApEinfo_fwdcolor=#800040 /ApEinfo_revcolor=#800040 misc_feature 6949..6969 /ApEinfo_label=AttB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd gene 899..2594 /ApEinfo_label=ura4+(intact) /ApEinfo_fwdcolor=#ffff66 /ApEinfo_revcolor=#ffff66 CDS 8693..8716 /ApEinfo_label=FLAG epitope(C2) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 CDS 8275..8484 /ApEinfo_label=ccdB ORF /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow CDS 1422..2216 /ApEinfo_label=ura4 ORF(intact) /ApEinfo_fwdcolor=#ffcc66 /ApEinfo_revcolor=#ffcc66 CDS 8717..8734 /ApEinfo_label=C-terminal hexahistidine tag /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 misc_feature complement(8638..8644) /ApEinfo_label=Gateway Recomb2 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 CDS 3876..4535 /ApEinfo_label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow terminator 1..237 /ApEinfo_label=ADH1 terminator /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 rep_origin 2836..3291 /ApEinfo_label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 4633..5315 /ApEinfo_label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 promoter 5747..6915 /ApEinfo_label=nmt1 promoter /ApEinfo_fwdcolor=#ff6fcf /ApEinfo_revcolor=#ff6fcf misc_feature 6956..6962 /ApEinfo_label=Gateway Recomb1 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 ORIGIN 1 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 61 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 121 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG 181 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 241 AGTCGTAGCT GCTAAAGGTG AGGGCTCCTA TTTGTTTGAC AAGGAAGGTC GTAAATACAT 301 TGACTTCACC TCTGGCGTAG CTGTTACATC TTTGGGTCAT GCTCATCCTG AAGTTGCTAG 361 ACTTGCCGCT GATCAATGCT CCAAATTAGT CCATAGTAGC AACCTTTTCT ACAACGAGCC 421 TGCCATTGAG CTATCTAATG TCATTAATAA TTCTTTGGCA AAAAATAGCG GTATTGCTGG 481 CCCCACAAAA ATTTTCTTTG CCAATTGTGG TACTGAGGCC AATGAGACAG CCTTGAAGTT 541 CGCTCGTAAG GCGGCCGCGT TTGAGAAGTA TGGAGAAGGG AAGAGTCAAA TTGTTTACTT 601 TAATAACTCT TTCCACGGCC GTTCTTTGGG ATCTCTTAGT ATCACTGCGA ACCCCAAATA 661 TAAACGTGGT TTCCAACCAT TACTTCCTGA TGTCGTCCAA GCCGTCTATA ATGACCCTGC 721 ATCCATAGAG CAATTTGTCA ATGACAAAAC TGCGGCTGTT ATTGTCGAGC CCGTTCAAGG 781 TGAAGGTGGT ATTTGTCCTG CCAAGCCTGA ATTTTTGATT GCTTTGCGCA AGGCCTGTGA 841 TAAGGTTGGT GCTTCTTTAA TCTATGATGA AATTCAATGC GGACTTGGTC GCAGATCTAC 901 AAATCCCACT GGCTATATGT ATGCATTTGT GTTAAAAAAG TTTGTATAGA TTATTTAATC 961 TACTCAGCAT TCTTTCTCTA AATAGGAATT TGTTACTTAA TGGAGAAAAA AATGTTTCGA 1021 TTTACCTAGT GTATTTGTTT GTATACTCAC GTTTAATTTC AAACATCCAT TCTATCTTGT 1081 GTAATTTTTG GCATGGTGAA AAAGATAATC AGCCTTATAA TCTTTACAAA AGTAAGAAAT 1141 TCTGTAAATA AGCCTTAATG CCCTTGCTTT AAATTAAAAT GGTTCTTTTT CATGATAATG 1201 TTTGCACTTT GTGAATATAT TTTAGATAGT TCTGTGAGGT ATAATTAAGA TGTTTTAGAG 1261 ACTTATACAA TTTTGTCTTT ATAAATTCTT AATTGATTTT ACCATCCCAG TTTAACTATG 1321 CTTCGTCGGC ATCTCTGCAC ATGTCGTGTT TTCTTACCGT ATTGTCCTAC CAAGAACCTC 1381 TTTTTTGCTT GGATCGAAAT TAAAGGTTTA AAAGCAAAGT TATGGATGCT AGAGTATTTC 1441 AAAGCTATTC AGCTAGAGCT GAGGGGATGA AAAATCCCAT TGCCAAGGAA TTGTTGGCTT 1501 TGATGGAAGA AAAGCAAAGC AACTTGTCAG TCGCGGTCGA TTTGACGAAG AAATCCGAAA 1561 TCTTAGAATT GGTAGATAAA ATTGGACCCT ATGTCTGTGT TATCAAGACA CATATTGACG 1621 TTGTCGAGGA TTTCGACCAG GATATGGTAG AAAAACTGGT GGCCTTAGGT AAAAAGCATC 1681 GTTTTCTTAT CTTTGAGGAT CGCAAATTCG CAGACATTGG AAATACCGTC AAGCTACAAT 1741 ATGCATCTGG TGTGTACAAA ATTGCTTCTT GGGCTCATAT CACAAATTGC CATACAGTGC 1801 CAGGCGAGGG TATTATACAA GGCCTCAAAG AAGTTGGTTT ACCTTTGGGA CGTGGTCTCT 1861 TGCTTTTGGC TGAAATGTCT TCCAAAGGCT CTTTGGCTAC TGGTTCCTAC ACAGAGAAAA 1921 CCTTAGAATG GTTTGAGAAG CATACCGATT TTTGCTTTGG CTTTATAGCT GGTCGTCGAT 1981 TTCCTAACCT TCAAAGCGAC TACATAACTA TGTCCCCTGG TATCGGCTTG GATGTTAAAG 2041 GAGACGGGCT GGGACAGCAA TATCGTACTC CTGAAGAAGT GATTGTAAAC TGCGGTAGCG 2101 ATATCATCAT TGTTGGTCGT GGAGTCTATG GAGCTGGTCG TAATCCTGTT GTCGAAGCCA 2161 AGAGATATAG AGAAGCTGGT TGGAAGGCAT ATCAGCAAAG ACTTTCTCAG CATTAAAAAA 2221 AGACTAATGT AAAATTTTTT TGGTTGGTTA TTGAAAAAGT CGATGCCTTG TTTGCGTTTG 2281 TTTTCCTAGG CGTTTTATGT CAGAAGGCAT TTAGAATTAG TATACAAGTA CTCTTTGGTA 2341 AAATTTTATG TAGCGACTAA AATATTAACT ATTATAGATA AACACCTTGG GAATAAAAAG 2401 TAATTTGCTA TAGTAATTTA TTAAACATGC TCCTACAACA TTACCACAAT CTTTTCTCTT 2461 GGATTGACAT TGAATAAGAA AAGAGTGAAT TTTTTTAGAC TTGTAATGAT AACTATGTAC 2521 AAAGCCAATG AAAGATGTAT GTAGATGAAT GTAAAATACC ATGTAGACAA ACAAGATAAA 2581 AATCACGTTT AAACGAGCTC GAATTCACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA 2641 AACCCTGGCG TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT 2701 AATAGCGAAG AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA 2761 TGGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT 2821 CAAAGCAACC ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA 2881 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC 2941 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT 3001 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG 3061 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA 3121 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT 3181 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA 3241 TTTAACAAAA ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA 3301 CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC 3361 CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA 3421 CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC 3481 GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT 3541 AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT 3601 AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT 3661 ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG 3721 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 3781 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC 3841 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT 3901 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT 3961 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA 4021 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 4081 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG 4141 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG 4201 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG 4261 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG 4321 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 4381 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC 4441 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA 4501 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT 4561 ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC 4621 TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG 4681 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT 4741 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC 4801 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC 4861 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG 4921 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT 4981 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT 5041 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC 5101 TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA 5161 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA 5221 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG 5281 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT 5341 GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA 5401 CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG 5461 TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA 5521 TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG 5581 CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG 5641 CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC 5701 ATGATTACGC CAAGCTTGCA TGCCTGCAGG TCGATCGACT CTAGAGGATC AGAAAATTAT 5761 CGCCATAAAA GACAGAATAA GTCATCAGCG GTTGTTTCAT TTCCTATATT TTTTTTTTAT 5821 TTTTTTATTT TTTAATAAGG GAAAATTTAA CGTCTAAGGA TACAGAAGAT TGTTAGCACA 5881 TTAAAGTAAT AAAGGCTTAA GTAGTAAGTG CCTTAGCATG TTATTGTATT TCAAAGGACA 5941 TAATCTAAAA TAATAACAAT ATCATTTCTC ACAAGTTATT CAATTTTCTT TTTTTTTTCT 6001 AATAATATCA AGAATGTATT ATTTGTTTGA CATAAGTCAA CTAATTTATT TAATATGCTG 6061 GATTAATCTT GCAGACATGT AAATTAACAA GTTTTAGTCA AATAACGTTG AAGTTTCAAT 6121 GAACTCAAAT AATTTCTCTT TTTTTTTATA TAACTCTAAT CTGATTTATA TTTTCCGCAG 6181 GGATCAACTG AAGTTATGAC ATTTGGATTG GATCACTTAT AACCTTGGTC GCCAAATAAT 6241 ACAAAAATCA GCGTTATAAA ACAAAGAAGG TTTTTGTTAA GAAATTAATC CTCTTTCTTG 6301 ATAAGAAAGT TGAACCGAAA TTGCAGATAC TGATATATGA AAATAATACC CACAATTTTG 6361 GGAATAGCGC AAGCCTCAAT TTAAACAATA GGTGAGGACA CATGATAATG ACCTCAATGA 6421 TTGTTAGAAG AAAAGAGCCT CATTACAAAA TCGAAAAATG AATGGTTGGG TACAAGTTTC 6481 CAAAACATGG TAAAGTGGAC TTTGCGTATG AGACGTAAAT AGAAAAAAAC ACTTGTTATA 6541 TGTTTTCTAG AATTATTGTT GTCTCTTTAT GGTTGGATGA TGCAAAATAG TAATTTCGGT 6601 TAGTTGCTGT AAAACACCAC GAGACAAATA GATATGGATA TTTATTAAAT CAGGAAAAAC 6661 GTAACTCTCG GCTACTGGAT GGTTCAGTCA CCCAACGATT ACTGGGGAGA GAAAACAGGG 6721 CAAAAGCAAA GCTTAAAGGA ATCCGATTGT CATTCGGCAA TGTGCAGCGA AACTAAAAAC 6781 CGGATAATGG ACCTGTTAAT CGAAACATTG AAGATATATA AAGGAAGAGG AATCCTGGCA 6841 TATCATCAAT TGAATAAGTT GAATTAATTA TTTCAATCTC ATTCTCACTT TCTGACTTAT 6901 AGTCGCTTTG TTAAAGCTAG CGTCGACAGG CCTGGATCCG GATATCAACA AGTTTGTACA 6961 AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC 7021 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC 7081 ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG 7141 TTAGGATCCG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG 7201 ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC 7261 AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC 7321 CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT 7381 GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG 7441 TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG 7501 TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA 7561 CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC 7621 CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT 7681 CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT 7741 GGCGATTCAG GTTCATCATG CCGTCTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA 7801 ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA AGATCTGGAT CCGGCTTACT 7861 AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTGATTTT TGCGGTATAA GAATATATAC 7921 TGATATGTAT ACCCGAAGTA TGTCAAAAAG AGGTGTGCTA TGAAGCAGCG TATTACAGTG 7981 ACAGTTGACA GCGACAGCTA TCAGTTGCTC AAGGCATATA TGATGTCAAT ATCTCCGGTC 8041 TGGTAAGCAC AACCATGCAG AATGAAGCCC GTCGTCTGCG TGCCGAACGC TGGAAAGCGG 8101 AAAATCAGGA AGGGATGGCT GAGGTCGCCC GGTTTATTGA AATGAACGGC TCTTTTGCTG 8161 ACGAGAACAG GGACTGGTGA AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT 8221 TATCGTCTGT TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATGGTG 8281 ATCCCCCTGG CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG 8341 GTGCATATCG GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC 8401 TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC 8461 ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG CCAGTCTGCA 8521 GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG 8581 CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTCTT 8641 GTACAAAGTG GTTGATATCA TGGATTATAA GGATGATGAC GATAAACCCA TGGACTACAA 8701 GGACGACGAT GATAAACATC ACCACCATCA CCACTGAGAT CTCGACTCTA GAGGATCC //