LOCUS 9185 bp ds-DNA circular 07-MAY-2008 DEFINITION ACCESSION VERSION SOURCE ORGANISM COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 241..552 /ApEinfo_label=arg1 5'-fragment /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff CDS 6730..7443 /ApEinfo_label=GFP(S2G/S65C) /ApEinfo_fwdcolor=#00ff80 /ApEinfo_revcolor=#00ff80 CDS 6682..6699 /ApEinfo_label=N-terminal hexahistidine tag /ApEinfo_fwdcolor=#ff8000 /ApEinfo_revcolor=#ff8000 promoter 5485..6653 /ApEinfo_label=nmt1 promoter /ApEinfo_fwdcolor=#ff6fcf /ApEinfo_revcolor=#ff6fcf misc_feature complement(9140..9146) /ApEinfo_label=Gateway Recomb2 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 CDS 557..894 /ApEinfo_label=arg1 3'-fragment /ApEinfo_fwdcolor=#6666ff /ApEinfo_revcolor=#6666ff CDS 6703..6726 /ApEinfo_label=FLAG epitope(1) /ApEinfo_fwdcolor=#008040 /ApEinfo_revcolor=#008040 misc_feature complement(9133..9153) /ApEinfo_label=AttB2 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd misc_feature 7458..7464 /ApEinfo_label=Gateway Recomb1 /ApEinfo_fwdcolor=#008080 /ApEinfo_revcolor=#008080 CDS 1282..2091 /ApEinfo_label=kanMX6 /ApEinfo_fwdcolor=#400080 /ApEinfo_revcolor=#400080 misc_feature 7451..7471 /ApEinfo_label=AttB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd CDS 3614..4273 /ApEinfo_label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow CDS 7683..8342 /ApEinfo_label=CmR /ApEinfo_fwdcolor=#800040 /ApEinfo_revcolor=#800040 CDS 8777..8986 /ApEinfo_label=ccdB ORF /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow terminator 1..237 /ApEinfo_label=ADH1 terminator /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 rep_origin 2574..3029 /ApEinfo_label=M13 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 rep_origin 4371..5053 /ApEinfo_label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 ORIGIN 1 GGCGCGCCAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 61 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 121 TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGTATGAGG 181 TCGCTCTTAT TGACCACACC TCTACCGGCA GATCCGCTAG GGATAACAGG GTAATATACT 241 AGTCGTAGCT GCTAAAGGTG AGGGCTCCTA TTTGTTTGAC AAGGAAGGTC GTAAATACAT 301 TGACTTCACC TCTGGCGTAG CTGTTACATC TTTGGGTCAT GCTCATCCTG AAGTTGCTAG 361 ACTTGCCGCT GATCAATGCT CCAAATTAGT CCATAGTAGC AACCTTTTCT ACAACGAGCC 421 TGCCATTGAG CTATCTAATG TCATTAATAA TTCTTTGGCA AAAAATAGCG GTATTGCTGG 481 CCCCACAAAA ATTTTCTTTG CCAATTGTGG TACTGAGGCC AATGAGACAG CCTTGAAGTT 541 CGCTCGTAAG GCGGCCGCGT TTGAGAAGTA TGGAGAAGGG AAGAGTCAAA TTGTTTACTT 601 TAATAACTCT TTCCACGGCC GTTCTTTGGG ATCTCTTAGT ATCACTGCGA ACCCCAAATA 661 TAAACGTGGT TTCCAACCAT TACTTCCTGA TGTCGTCCAA GCCGTCTATA ATGACCCTGC 721 ATCCATAGAG CAATTTGTCA ATGACAAAAC TGCGGCTGTT ATTGTCGAGC CCGTTCAAGG 781 TGAAGGTGGT ATTTGTCCTG CCAAGCCTGA ATTTTTGATT GCTTTGCGCA AGGCCTGTGA 841 TAAGGTTGGT GCTTCTTTAA TCTATGATGA AATTCAATGC GGACTTGGTC GCAGATCTGT 901 TTAGCTTGCC TCGTCCCCGC CGGGTCACCC GGCCAGCGAC ATGGAGGCCC AGAATACCCT 961 CCTTGACAGT CTTGACGTGC GCAGCTCAGG GGCATGATGT GACTGTCGCC CGTACATTTA 1021 GCCCATACAT CCCCATGTAT AATCATTTGC ATCCATACAT TTTGATGGCC GCACGGCGCG 1081 AAGCAAAAAT TACGGCTCCT CGCTGCAGAC CTGCGAGCAG GGAAACGCTC CCCTCACAGA 1141 CGCGTTGAAT TGTCCCCACG CCGCGCCCCT GTAGAGAAAT ATAAAAGGTT AGGATTTGCC 1201 ACTGAGGTTC TTCTTTCATA TACTTCCTTT TAAAATCTTG CTAGGATACA GTTCTCACAT 1261 CACATCCGAA CATAAACAAC CATGGGTAAG GAAAAGACTC ACGTTTCGAG GCCGCGATTA 1321 AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA 1381 TCAGGTGCGA CAATCTATCG ATTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA 1441 CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG 1501 ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG 1561 TTACTCACCA CTGCGATCCC CGGCAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT 1621 TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT 1681 GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA 1741 ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT 1801 GAACAAGTCT GGAAAGAAAT GCATAAGCTT TTGCCATTCT CACCGGATTC AGTCGTCACT 1861 CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT 1921 GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC 1981 CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT 2041 CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGTACTG 2101 ACAATAAAAA GATTCTTGTT TTCAAGAACT TGTCATTTGT ATAGTTTTTT TATATTGTAG 2161 TTGTTCTATT TTAATCAAAT GTTAGCGTGA TTTATATTTT TTTTCGCCTC GACATCATCT 2221 GCCCAGATGC GAAGTTAAGT GCGCAGAAAG TAATATCATG CGTCAATCGT ATGTGAATGC 2281 TGGTCGCTAT ACTGCTGTCG ATTCGATACT AACGCCGCCA TCCAGTTTAA ACGAGCTCGA 2341 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA 2401 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG 2461 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC 2521 TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT AGTACGCGCC 2581 CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT 2641 TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC 2701 CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT 2761 ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG GGCCATCGCC 2821 CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT 2881 GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT TATAAGGGAT 2941 TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA 3001 TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA TCTGCTCTGA 3061 TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC 3121 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG 3181 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG TGATACGCCT 3241 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 3301 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 3361 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 3421 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 3481 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 3541 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 3601 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 3661 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 3721 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 3781 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 3841 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 3901 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 3961 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 4021 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 4081 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 4141 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 4201 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 4261 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 4321 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 4381 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 4441 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 4501 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 4561 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 4621 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 4681 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 4741 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 4801 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC 4861 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 4921 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 4981 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 5041 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 5101 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 5161 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 5221 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 5281 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 5341 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 5401 GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG 5461 CCTGCAGGTC GATCGACTCT AGAGGATCAG AAAATTATCG CCATAAAAGA CAGAATAAGT 5521 CATCAGCGGT TGTTTCATTT CCTATATTTT TTTTTTATTT TTTTATTTTT TAATAAGGGA 5581 AAATTTAACG TCTAAGGATA CAGAAGATTG TTAGCACATT AAAGTAATAA AGGCTTAAGT 5641 AGTAAGTGCC TTAGCATGTT ATTGTATTTC AAAGGACATA ATCTAAAATA ATAACAATAT 5701 CATTTCTCAC AAGTTATTCA ATTTTCTTTT TTTTTTCTAA TAATATCAAG AATGTATTAT 5761 TTGTTTGACA TAAGTCAACT AATTTATTTA ATATGCTGGA TTAATCTTGC AGACATGTAA 5821 ATTAACAAGT TTTAGTCAAA TAACGTTGAA GTTTCAATGA ACTCAAATAA TTTCTCTTTT 5881 TTTTTATATA ACTCTAATCT GATTTATATT TTCCGCAGGG ATCAACTGAA GTTATGACAT 5941 TTGGATTGGA TCACTTATAA CCTTGGTCGC CAAATAATAC AAAAATCAGC GTTATAAAAC 6001 AAAGAAGGTT TTTGTTAAGA AATTAATCCT CTTTCTTGAT AAGAAAGTTG AACCGAAATT 6061 GCAGATACTG ATATATGAAA ATAATACCCA CAATTTTGGG AATAGCGCAA GCCTCAATTT 6121 AAACAATAGG TGAGGACACA TGATAATGAC CTCAATGATT GTTAGAAGAA AAGAGCCTCA 6181 TTACAAAATC GAAAAATGAA TGGTTGGGTA CAAGTTTCCA AAACATGGTA AAGTGGACTT 6241 TGCGTATGAG ACGTAAATAG AAAAAAACAC TTGTTATATG TTTTCTAGAA TTATTGTTGT 6301 CTCTTTATGG TTGGATGATG CAAAATAGTA ATTTCGGTTA GTTGCTGTAA AACACCACGA 6361 GACAAATAGA TATGGATATT TATTAAATCA GGAAAAACGT AACTCTCGGC TACTGGATGG 6421 TTCAGTCACC CAACGATTAC TGGGGAGAGA AAACAGGGCA AAAGCAAAGC TTAAAGGAAT 6481 CCGATTGTCA TTCGGCAATG TGCAGCGAAA CTAAAAACCG GATAATGGAC CTGTTAATCG 6541 AAACATTGAA GATATATAAA GGAAGAGGAA TCCTGGCATA TCATCAATTG AATAAGTTGA 6601 ATTAATTATT TCAATCTCAT TCTCACTTTC TGACTTATAG TCGCTTTGTT AAAGCTAGCG 6661 TCGACAGGCC TGGATCCTAT GCATCACCAC CATCATCATA TGGATTATAA GGATGATGAC 6721 GATAAACCCA TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT TCTTGTTGAA 6781 TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA AGGTGATGCA 6841 ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC TGTTCCGTGG 6901 CCAACACTTG TCACTACTTT CTGTTATGGT GTTCAATGCT TTTCAAGATA CCCAGATCAC 6961 ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GCTATGTACA GGAAAGAACT 7021 ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT TGAAGGTGAT 7081 ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG AAACATTCTT 7141 GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC AGACAAACAA 7201 AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG AAGCGTTCAA 7261 CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT TTTACCAGAC 7321 AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA GAGAGACCAC 7381 ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA TGAACTATAC 7441 AAAGATATCA CAAGTTTGTA CAAAAAAGCT GAACGAGAAA CGTAAAATGA TATAAATATC 7501 AATATATTAA ATTAGATTTT GCATAAAAAA CAGACTACAT AATACTGTAA AACACAACAT 7561 ATCCAGTCAC TATGGCGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT 7621 CGTATAATGT GTGGATTTTG AGTTAGGATC CGGCGAGATT TTCAGGAGCT AAGGAAGCTA 7681 AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG 7741 AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG 7801 ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA 7861 TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG 7921 GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG 7981 AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT 8041 ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG 8101 AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG 8161 TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG 8221 GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC 8281 ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT 8341 AAACGCGTGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT 8401 TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTGTGC 8461 TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA 8521 TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG 8581 CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT 8641 GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT TTAAGGTTTA 8701 CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA 8761 CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT 8821 CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC 8881 CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG 8941 CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC 9001 CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT 9061 ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT 9121 ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTGATATC ATGAGATCTC GACTCTAGAG 9181 GATCC //