| Gene name |
SPAC212.04c |
| Gene ID |
52/D12 |
| Gene synonyms/obsolete |
|
| Gene product |
S. pombe specific
families |
| Entry clone |
Cloned in 2004
trial |
| ORF length (unspliced) |
922 |
| ORF length (spliced) |
867 |
| Entry clone length |
922 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC212.02.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTGCATTAATGAACCA |
| Rev primer name |
SPAC212.02.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGATCTCAGTAAGACACGCT |
| Amino acid length |
288 |
| Molecular weight |
32.2 |
| Isoelectric point (calc.) |
8.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
|
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
|
| Localization (YFP) |
cytoplasmic dots;
ER |
| Comments for localization |
bright dots by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |