| Gene name |
SPAC17G8.01c |
| Gene ID |
52/B07 |
| Gene synonyms/obsolete |
SPAC6C3.10c |
| Gene product |
tRNA ligase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2411 |
| ORF length (spliced) |
2364 |
| Entry clone length |
2364 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17G8.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTCGTGATCAAAGAGT |
| Rev primer name |
SPAC17G8.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGTAAACGGGTTCCAGCATT |
| Amino acid length |
787 |
| Molecular weight |
90.3 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol, partially |
| Microscope used for
observation |
Zeiss |