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Schizosaccharomyces pombe Postgenome Database

Gene name SPAP7G5.06
Gene ID 52/B05
Gene synonyms/obsolete
Gene product amino acid permease family; similar to Sp SPAC869.11 and SPBC359.01 and meu22 and SPBPB2B2.01 and isp5
Entry clone Cloned
ORF length (unspliced) 1752
ORF length (spliced)
Entry clone length 1752
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAP7G5.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCGTCGCCAGATCGCTC
Rev primer name SPAP7G5.06.Rv
Rev primer SEQ AGAAAGCTGGGTAGCAAAGAGATTGCCAAATC
Amino acid length 583
Molecular weight 64
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi; periphery at cell tip
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Zeiss

Image information
YFP 4 images) See all images

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