| Gene name |
SPAC11E3.04c |
| Gene ID |
52/A04 |
| Gene synonyms/obsolete |
ubc13; spu13 |
| Gene product |
ubiquitin conjugating
enzyme; involved in ubiquitination (lys63); involved in DNA
repair; involved in DNA postreplication repair |
| Entry clone |
Cloned |
| ORF length (unspliced) |
698 |
| ORF length (spliced) |
447 |
| Entry clone length |
698 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC11E3.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTTTGCCTAAGCGTAT |
| Rev primer name |
SPAC11E3.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACTGCATACTTTTTCGTC |
| Amino acid length |
148 |
| Molecular weight |
16.8 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Zeiss |