Gene name |
SPAPB18E9.01 |
Gene ID |
51/A02 |
Gene synonyms/obsolete |
|
Gene product |
tRNA (guanine)
methyltransferase activity; involved in tRNA methylation
|
Entry clone |
Cloned |
ORF length (unspliced) |
1674 |
ORF length (spliced) |
1308 |
Entry clone length |
1674 |
No. of intron |
6 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAPB18E9.01.Fd |
Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCATTTACAGATTAGAAC |
Rev primer name |
SPAPB18E9.01.Rv |
Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTATATAAAGAAGGATATTACC |
Amino acid length |
435 |
Molecular weight |
50.2 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Zeiss |