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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1348.14c
Gene ID 50/H01
Gene synonyms/obsolete SPAC1348.14c; SPAPB8B6.01c; SPAC1348.14; SPAP8B6.01c
Gene product hexose transporter; ght7?; similar to Sp GHT1 and GHT2 and GHT4 and GHT5 and GHT6 and SPCC548.06C and GHT3
Entry clone Cloned
ORF length (unspliced) 1557
ORF length (spliced)
Entry clone length 1557
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAP8B6.01.Fd
Fwd primer SEQ GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGCGCGATTTTCAATCACG
Rev primer name SPAP8B6.01.Rv
Rev primer SEQ GGGGACCACTTTGTACAAGAAAGCTGGGTAAAGACGAGGCTCCCGTTTA
Amino acid length 518
Molecular weight 57.9
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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