| Gene name |
SPBC11B10.05c |
| Gene ID |
50/G05 |
| Gene synonyms/obsolete |
pi006;
SPACTOKYO_453.30c |
| Gene product |
random septum position
protein Rsp1; DNAJ domain protein; similar to S. pombe
SPBC1773.09C; no apparent orthologs; non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1485 |
| ORF length (spliced) |
|
| Entry clone length |
1485 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
50T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC11B10.05.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGCGAGGACGGCGTTTCA |
| Rev primer name |
SPBC11B10.05.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTATATACCAAAAGATGCGAAA |
| Amino acid length |
494 |
| Molecular weight |
56.2 |
| Isoelectric point (calc.) |
9.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQSILRSLAI |
| Localization (YFP) |
SPB; cytoplasmic dots;
site of septum formation |
| Comments for localization |
microtubule-associated
dots |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |