| Gene name |
SPAC4A8.14 |
| Gene ID |
50/E09 |
| Gene synonyms/obsolete |
prs1 |
| Gene product |
ribose-phosphate
pyrophosphokinase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1272 |
| ORF length (spliced) |
1230 |
| Entry clone length |
1272 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
477C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4A8.14.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGAAGAGTGCTACAATCAT |
| Rev primer name |
SPAC4A8.14.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGAACGCTTTCTTAAACAAG |
| Amino acid length |
409 |
| Molecular weight |
45.3 |
| Isoelectric point (calc.) |
5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLHLISNRLGI |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |