| Gene name |
SPAC20H4.03c |
| Gene ID |
50/E02 |
| Gene synonyms/obsolete |
tfs1 |
| Gene product |
transcription
elongation factor SII |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1226 |
| ORF length (spliced) |
882 |
| Entry clone length |
1226 |
| No. of intron |
4 |
| Sequence status |
Partially
sequenced |
| Sequence results |
#Low sequence
quality |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC20H4.03.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGGACTCTGCTGATATCCG |
| Rev primer name |
SPAC20H4.03.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAAGAAAATTTCCAGCGATTT |
| Amino acid length |
293 |
| Molecular weight |
32.7 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |