| Gene name |
SPBPB10D8.04c |
| Gene ID |
50/D01 |
| Gene synonyms/obsolete |
|
| Gene product |
malate permease;
similar to Sp SPBPB10D8.05c and SPBPB10D8.06c and
SPBPB10D8.07c (paralogs); tandem duplication |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1140 |
| ORF length (spliced) |
|
| Entry clone length |
1140 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBPB10D8.04.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGTCTTGGTTTTACAGATT |
| Rev primer name |
SPBPB10D8.04.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTAGATAATACCAACCAAATTT |
| Amino acid length |
379 |
| Molecular weight |
43.1 |
| Isoelectric point (calc.) |
9.7 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
9 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVQSLILSLFL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |