| Gene name |
SPAC31G5.01 |
| Gene ID |
50/C03 |
| Gene synonyms/obsolete |
sap49;
SPAPB1A11.05 |
| Gene product |
RNA-binding protein;
rrm RNA recognition motif; involved in mRNA splicing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1065 |
| ORF length (spliced) |
1008 |
| Entry clone length |
1065 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
182T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC31G5.01.Fd |
| Fwd primer SEQ |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCTCATATGAGTATTCGAGAAGACAG |
| Rev primer name |
SPAC31G5.01.Rv |
| Rev primer SEQ |
GGGGACCACTTTGTACAAGAAAGCTGGGTACCTCCTTTGTTGTTGACTG |
| Amino acid length |
335 |
| Molecular weight |
35.9 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |