| Gene name |
SPAC823.01c |
| Gene ID |
49/F04 |
| Gene synonyms/obsolete |
tif33; SPCP1E11.01c;
SPAC4A8.16c |
| Gene product |
translation initiation
factor 3 (eif3 subunit); PCI domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2757 |
| ORF length (spliced) |
|
| Entry clone length |
2757 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
144A:T / 309T:C /
1047T:C / 2289T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC823.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGAGATTCTTCAAGGG |
| Rev primer name |
SPAC823.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTCATGTCTGAACGATTC |
| Amino acid length |
918 |
| Molecular weight |
104.3 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
507 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTELHSILDI/LPFHMHINLEL |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |