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Schizosaccharomyces pombe Postgenome Database

Gene name SPCPB1C11.03
Gene ID 49/B06
Gene synonyms/obsolete
Gene product membrane transporter; similar to Sp SPPB10D8.01 (paralog)
Entry clone Cloned
ORF length (unspliced) 1713
ORF length (spliced)
Entry clone length 1713
No. of intron 0
Sequence status Finished
Sequence results 331A:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCPB1C11.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACCGAGTCTATCATCTC
Rev primer name SPCPB1C11.03.Rv
Rev primer SEQ AGAAAGCTGGGTAAAGAGGATAAACAAAGTTT
Amino acid length 570
Molecular weight 64.3
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain 10
NLS position (Columbia Univ. Bioinformatics Center) 510
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LKWKLYIYLLL/LGFLDMMLFI
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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