| Gene name |
SPBC691.04 |
| Gene ID |
49/A07 |
| Gene synonyms/obsolete |
pi031;
SPACTOKYO_453.03c |
| Gene product |
DEAD/DEAH box
helicase; ATP-dependent; RNA helicase; similar to Sp
SPAC1F7.02C (paralog) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1608 |
| ORF length (spliced) |
|
| Entry clone length |
1608 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
841T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC691.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAATATCTCACTGAAAGG |
| Rev primer name |
SPBC691.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAGCGACGACTTTGTTGATAG |
| Amino acid length |
535 |
| Molecular weight |
59.4 |
| Isoelectric point (calc.) |
10.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDKVFSSSLSL/LNSIKHLPL |
| Localization (YFP) |
mitochondrion? |
| Comments for localization |
aggregates |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |