Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB2B2.09c
Gene ID 49/A04
Gene synonyms/obsolete
Gene product ketopantoate reductase
Entry clone Cloned
ORF length (unspliced) 1053
ORF length (spliced)
Entry clone length 1053
No. of intron 0
Sequence status Finished
Sequence results 134A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBPB2B2.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAATAACACAATTTATAT
Rev primer name SPBPB2B2.09.Rv
Rev primer SEQ AGAAAGCTGGGTATCGTGCATGCGCTCGACAT
Amino acid length 350
Molecular weight 39.1
Isoelectric point (calc.) 8.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.