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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC6G9.04
Gene ID 48/F06
Gene synonyms/obsolete
Gene product pleckstrin homology domain
Entry clone Cloned
ORF length (unspliced) 3957
ORF length (spliced)
Entry clone length 3957
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC6G9.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATTTTAGAATAACAGA
Rev primer name SPAC6G9.04.Rv
Rev primer SEQ AGAAAGCTGGGTATAATCCTTTAAAATCATAA
Amino acid length 1318
Molecular weight 150.9
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LVSISKLLL
Localization (YFP) nuclear envelope; spindle microtubules; nucleusGSPB
Comments for localization nuclear dots by over expression; periphery by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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