Gene name |
SPCC5E4.06 |
Gene ID |
48/D06 |
Gene synonyms/obsolete |
rad18 |
Gene product |
involved in DNA
repair; involved in DNA damage response (required); SMC6
complex |
Entry clone |
Cloned |
ORF length (unspliced) |
3529 |
ORF length (spliced) |
3423 |
Entry clone length |
3529 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC5E4.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTACAGAGCTTACTAA |
Rev primer name |
SPCC5E4.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGCGCCGTAGAGGGAGGA |
Amino acid length |
1140 |
Molecular weight |
130.9 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHTLINHLGI |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |