| Gene name |
SPAC17A5.16 |
| Gene ID |
48/B06 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved eukaryotic
protein; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3255 |
| ORF length (spliced) |
2778 |
| Entry clone length |
3255 |
| No. of intron |
7 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC17A5.16.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGAGGCCAAGAGTCAAA |
| Rev primer name |
SPAC17A5.16.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTGTTGCGTAAACTGGAA |
| Amino acid length |
925 |
| Molecular weight |
104.8 |
| Isoelectric point (calc.) |
6.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYSLINTTLRL/LTSQILLLFL/LSPVILLTI/LDKLDLLYL |
| Localization (YFP) |
Golgi; cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus; Golgi |
| Microscope used for
observation |
Leica |