Gene name |
SPAC1F3.03 |
Gene ID |
47/H08 |
Gene synonyms/obsolete |
|
Gene product |
WD repeat protein;
involved in exocytosis; Sc sro7/sro77 function downstream of
the Rho3p GTPase |
Entry clone |
Cloned |
ORF length (unspliced) |
3153 |
ORF length (spliced) |
3015 |
Entry clone length |
3153 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC1F3.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGCTTTTTTAAAAAAAA |
Rev primer name |
SPAC1F3.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGTATATAGCTTTTTAAG |
Amino acid length |
1004 |
Molecular weight |
112 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots at
cell tip and site of septum formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |