Gene name |
SPACUNK4.12c |
Gene ID |
47/D09 |
Gene synonyms/obsolete |
|
Gene product |
peptidase family M16;
involved in pheromone response |
Entry clone |
Cloned |
ORF length (unspliced) |
2910 |
ORF length (spliced) |
|
Entry clone length |
2910 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPACUNK4.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATGGGAAACCGCAAGT |
Rev primer name |
SPACUNK4.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATTAAATAATAAAGACCTA |
Amino acid length |
969 |
Molecular weight |
112.1 |
Isoelectric point (calc.) |
5.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGDVPKELGL/LAYLKSLGL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |