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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC57A7.06
Gene ID 47/C08
Gene synonyms/obsolete
Gene product ribonucleoprotein (RNP) complex; small subunit (SSU) processome component; involved in rRNA processing; predicted coiled-coil protein; predicted ATP/GTP binding (prosite)
Entry clone Cloned
ORF length (unspliced) 2836
ORF length (spliced) 2790
Entry clone length 2836
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC57A7.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTAGGAAAGGAAAAGT
Rev primer name SPAC57A7.06.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAGGTGCCTTAATAGGA
Amino acid length 929
Molecular weight 105
Isoelectric point (calc.) 4.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 237
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>>nucleus?
Comments for localization aggregates
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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