| Gene name |
SPAC19G12.01c |
| Gene ID |
46/H03 |
| Gene synonyms/obsolete |
cut20; lid1;
SPAPJ698.04c |
| Gene product |
cyclosome/apc subunit;
Cut20/Apc4 anaphase-promoting complex (APC); cyclosome; WD
repeat protein (putative); involved in metaphase-anaphase
transition (regulation) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2386 |
| ORF length (spliced) |
2160 |
| Entry clone length |
2386 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPAC19G12.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTGTCAAAATCTTTCAA |
| Rev primer name |
SPAC19G12.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAAGAGAATAAACGATAT |
| Amino acid length |
719 |
| Molecular weight |
82.6 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB?; nuclear dots;
nucleus>cytosol; periphery at site of septum
formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |