| Gene name |
SPBC243.01 |
| Gene ID |
46/D02 |
| Gene synonyms/obsolete |
mph1; SPBC1271.16c;
SPBC106.01 |
| Gene product |
yeast spindle
checkpoint protein kinase Mps Mps1-like family;
serine/threonine protein kinase; dual specificity protein
kinase; involved in spindle assembly checkpoint (required);
non-essential |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2037 |
| ORF length (spliced) |
|
| Entry clone length |
2037 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC243.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAAGCGCAATCCTCC |
| Rev primer name |
SPBC243.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTGGCATTTTTCGTAAA |
| Amino acid length |
678 |
| Molecular weight |
75.3 |
| Isoelectric point (calc.) |
7.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |