Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC20G8.04c
Gene ID 46/B01
Gene synonyms/obsolete
Gene product electron transfer flavoprotein-ubiquinone oxidoreductase
Entry clone Cloned
ORF length (unspliced) 1899
ORF length (spliced)
Entry clone length 1899
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC20G8.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTATTTATCGAGATC
Rev primer name SPAC20G8.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGGTCAAGGTATATTTAGGG
Amino acid length 632
Molecular weight 69.4
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER?
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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