| Gene name |
SPBC646.04 |
| Gene ID |
45/H12 |
| Gene synonyms/obsolete |
pla1 |
| Gene product |
poly(A) polymerase;
nucleotidyltransferase; involved in polyadenylation |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1824 |
| ORF length (spliced) |
1701 |
| Entry clone length |
1824 |
| No. of intron |
3 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC646.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGACTACCAAGCAATGGGG |
| Rev primer name |
SPBC646.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGCCGTTGAAACTTTTTGT |
| Amino acid length |
566 |
| Molecular weight |
64.1 |
| Isoelectric point (calc.) |
8.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRHLVTRLEL |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal |