Gene name |
SPBC1773.12 |
Gene ID |
45/H01 |
Gene synonyms/obsolete |
|
Gene product |
transcriptional
regulator; zinc finger protein; zf-fungal Zn(2)-Cys(6)
binuclear cluster domain |
Entry clone |
Cloned |
ORF length (unspliced) |
1785 |
ORF length (spliced) |
|
Entry clone length |
1785 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1773.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTACCAATAAAAAAAAT |
Rev primer name |
SPBC1773.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACACCAACAAACTCTCCAGT |
Amino acid length |
594 |
Molecular weight |
69.4 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
40 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDKIQALTI |
Localization (YFP) |
nucleolus>nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |