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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC359.03c
Gene ID 45/F12
Gene synonyms/obsolete
Gene product amino acid permease
Entry clone Cloned
ORF length (unspliced) 1740
ORF length (spliced)
Entry clone length 1740
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC359.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGCTAAAGATTATGA
Rev primer name SPBC359.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGCAAACGGTATTAATTATT
Amino acid length 579
Molecular weight 63.2
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain 11
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi; periphery at cell tip
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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