Gene name |
SPBC418.01c |
Gene ID |
45/F09 |
Gene synonyms/obsolete |
his4;
SPBC887.20c |
Gene product |
bifunctional enzyme;
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)
methylideneamino]imidazole-4-carboxamide isomerase; involved
in histidine biosynthesis (4th step); glutamine
amidotransferase |
Entry clone |
Cloned in 2004
trial |
ORF length (unspliced) |
1732 |
ORF length (spliced) |
1626 |
Entry clone length |
1732 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC418.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGTTTCAATAGTTGA |
Rev primer name |
SPBC418.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGTGCGAACCAGAACGTCG |
Amino acid length |
541 |
Molecular weight |
59.3 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |