Gene name |
SPCC63.02c |
Gene ID |
45/E11 |
Gene synonyms/obsolete |
|
Gene product |
glycosyl hydrolase
family 13; alpha-amylase; GPI anchored protein; glycoprotein;
no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1695 |
ORF length (spliced) |
|
Entry clone length |
1695 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC63.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTGGTGTTTATTTTGT |
Rev primer name |
SPCC63.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGAAGAATAAAACAAAGAGC |
Amino acid length |
564 |
Molecular weight |
63.2 |
Isoelectric point (calc.) |
4 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |