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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC13E7.02
Gene ID 45/C09
Gene synonyms/obsolete cwf24
Gene product acetyltransferase; GNAT family; complexed with Cdc5p; zinc finger protein; zf-C3HC4 type (RING finger); ubiquitin ligase (E3); zf-CCCH type; no apparent orthologs (with acetyltransferase domain, possible deletion? check sequence*)
Entry clone Cloned#
ORF length (unspliced) 1602
ORF length (spliced)
Entry clone length 1602
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPBC13E7.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACAAAAAAATTTAAA
Rev primer name SPBC13E7.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGATTTGAAATTATATTA
Amino acid length 533
Molecular weight 60.7
Isoelectric point (calc.) 9.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 27/29/68
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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