Gene name |
SPBC1861.09 |
Gene ID |
45/C02 |
Gene synonyms/obsolete |
|
Gene product |
ole inferred from
homology; serine/threonine protein kinase |
Entry clone |
Cloned# |
ORF length (unspliced) |
1581 |
ORF length (spliced) |
|
Entry clone length |
1581 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPBC1861.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTCGAGAAACTGAATT |
Rev primer name |
SPBC1861.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCATCACCAGCATGATGA |
Amino acid length |
526 |
Molecular weight |
59.8 |
Isoelectric point (calc.) |
7.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol; periphery,
especially at cell tip and site of septum formation;
cytoplasmic dots |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus>>cytosol) |
Microscope used for
observation |
DeltaVision |