Gene name |
SPAC5D6.04 |
Gene ID |
45/B12 |
Gene synonyms/obsolete |
|
Gene product |
membrane transporter;
unknown specificity |
Entry clone |
Cloned |
ORF length (unspliced) |
1581 |
ORF length (spliced) |
1359 |
Entry clone length |
1581 |
No. of intron |
3 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC5D6.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGATTCTTTTCTTCTCT |
Rev primer name |
SPAC5D6.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTGTCCATTTCACAACT |
Amino acid length |
452 |
Molecular weight |
49.5 |
Isoelectric point (calc.) |
4.9 |
Signal SEQ |
|
No. of transmembrane domain |
6 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNLKMLIDLSL/LYSLFIALFI/LGRMVVVPLAL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |