| Gene name |
SPCC777.09c |
| Gene ID |
45/B10 |
| Gene synonyms/obsolete |
arg1 |
| Gene product |
acetylornithine
aminotransferase; involved in ornithine biosynthesis; involved
in arginine biosynthesis via ornithine; involved in glutamate
catabolism; acetylornithine aminotransferase activity;
functionally complements arg1-230 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1574 |
| ORF length (spliced) |
1326 |
| Entry clone length |
1574 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPCC777.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTTCAAAGACGATCACT |
| Rev primer name |
SPCC777.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTACTTAAAGCCTTCAAA |
| Amino acid length |
441 |
| Molecular weight |
47.5 |
| Isoelectric point (calc.) |
9.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
mitochondrion;
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |