Gene name |
SPCC553.12c |
Gene ID |
45/B03 |
Gene synonyms/obsolete |
SPCC794.13 |
Gene product |
low similarity to NADH
dehydrogenase subunit 2 by multiple alignment; no apparent Sc
ortholog |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1566 |
ORF length (spliced) |
|
Entry clone length |
1566 |
No. of intron |
0 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPCC553.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGGATAGTTTTGATGC |
Rev primer name |
SPCC553.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGTGAAGACTTATTATA |
Amino acid length |
521 |
Molecular weight |
60.5 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
10 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSNFVLRNLML |
Localization (YFP) |
no expression
clone |
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|