Gene name |
SPAC3C7.07c |
Gene ID |
44/F12 |
Gene synonyms/obsolete |
|
Gene product |
arginine-tRNA protein
transferase |
Entry clone |
Cloned |
ORF length (unspliced) |
1451 |
ORF length (spliced) |
1086 |
Entry clone length |
1451 |
No. of intron |
6 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC3C7.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTATTGGATAAACTGTT |
Rev primer name |
SPAC3C7.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACTAGATAAAACAAGTGTA |
Amino acid length |
361 |
Molecular weight |
41.7 |
Isoelectric point (calc.) |
8.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSRLKRLVL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |