Gene name |
SPAC25B8.06c |
Gene ID |
44/F01 |
Gene synonyms/obsolete |
|
Gene product |
probable seryl-tRNA
synthetase; serine-tRNA ligase; involved in seryl-tRNA
aminoacylation; serine-tRNA ligase activity; cellular
component unknown |
Entry clone |
Cloned |
ORF length (unspliced) |
1422 |
ORF length (spliced) |
1365 |
Entry clone length |
1422 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC25B8.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTCAATTGTTGTATTT |
Rev primer name |
SPAC25B8.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTTGCTTTAAAAAGGTAT |
Amino acid length |
454 |
Molecular weight |
51.2 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKNEFFPLLSL |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |