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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC18B5.06
Gene ID 44/D11
Gene synonyms/obsolete erf1; sup45
Gene product translation release factor; eRF1-like protein
Entry clone Cloned
ORF length (unspliced) 1390
ORF length (spliced) 1173
Entry clone length 1390
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC18B5.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAGTTGATTCAAAAAAA
Rev primer name SPCC18B5.06.Rv
Rev primer SEQ AGAAAGCTGGGTAACTATGTTCAAAATTTTGG
Amino acid length 390
Molecular weight 44.3
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGAIGELLI
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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