Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4G3.07c
Gene ID 44/D07
Gene synonyms/obsolete
Gene product zinc finger protein; transcriptional regulator; zf-PHD finger; similar to human retinoblastoma binding protein 2; similar toSp SPAC30D11.08c (paralog); no apparentSc ortholog; involved in transcriptional regulation
Entry clone Cloned
ORF length (unspliced) 1386
ORF length (spliced)
Entry clone length 1386
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC4G3.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCAAAAGAATTTTTT
Rev primer name SPCC4G3.07.Rv
Rev primer SEQ AGAAAGCTGGGTATAAAACAGTAGCACATAAA
Amino acid length 461
Molecular weight 51.2
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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