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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC4B3.05c
Gene ID 44/B02
Gene synonyms/obsolete
Gene product uroporphyrinogen decarboxylase
Entry clone Cloned (also cloned in 2004 trial)
ORF length (unspliced) 1267
ORF length (spliced) 1113
Entry clone length 1267
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC4B3.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAAATGACTTGATTCT
Rev primer name SPCC4B3.05.Rv
Rev primer SEQ AGAAAGCTGGGTACGCAGAGCCATATTGATGA
Amino acid length 370
Molecular weight 41.8
Isoelectric point (calc.) 6.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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